Target (protein/gene name): 2-oxoglutarate dehydrogenase complex NCBI Gene # or RefSeq#: 3266428 Protein ID (NP or XP #) or Wolbachia#: Q5GSP1 Organism: Wolbachia Etiologic Risk Group (see link below): N/A Background/Disease Information: Essentiality of this protein:http://tdrtargets.org/targets/view?gene_id=232973 Complex of enzymes: EC#: 1.2.4.2 Link to BRENDA EC# page:http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.2.4.2 Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): -- link to Sigma (or other company) page for assay or assay reagents (substrates) -- link to paper that contains assay information Structure Available (PDB or Homology model):http://www.rcsb.org/pdb/explore/explore.do?structureId=2JGD -- PDB # or closest PDB entry if using homology model: 2JGD -- Query Coverage (if not direct match): 98% -- Max Ident (if not direct match): 42% Druggable Target (see Databases for this): Current Inhibitors: Expression Information (has it been expressed in bacterial cells): Purification Method: Image of protein (PyMol or etc): Amino Acid Sequence: 887 Wolbachia; 933 E. Coli length of your protein in Amino Acids Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website: E. Coli: 105,062 Da Wolbachia: 100,186 Da Molar Extinction coefficient of your protein at 280 nm wavelength: CDS Gene Sequence: GC% Content for gene: CDS Gene Sequence (codon optimized) - after having done Primer Design: GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):
NCBI Gene # or RefSeq#: 3266428
Protein ID (NP or XP #) or Wolbachia#: Q5GSP1
Organism: Wolbachia
Etiologic Risk Group (see link below): N/A
Background/Disease Information:
Essentiality of this protein: http://tdrtargets.org/targets/view?gene_id=232973
Complex of enzymes:
EC#: 1.2.4.2
Link to BRENDA EC# page: http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.2.4.2
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay or assay reagents (substrates)
-- link to paper that contains assay information
Structure Available (PDB or Homology model): http://www.rcsb.org/pdb/explore/explore.do?structureId=2JGD
-- PDB # or closest PDB entry if using homology model: 2JGD
-- Query Coverage (if not direct match): 98%
-- Max Ident (if not direct match): 42%
Druggable Target (see Databases for this):
Current Inhibitors:
Expression Information (has it been expressed in bacterial cells):
Purification Method:
Image of protein (PyMol or etc):
Amino Acid Sequence: 887 Wolbachia; 933 E. Coli
length of your protein in Amino Acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website: E. Coli: 105,062 Da Wolbachia: 100,186 Da
Molar Extinction coefficient of your protein at 280 nm wavelength:
CDS Gene Sequence:
GC% Content for gene:
CDS Gene Sequence (codon optimized) - after having done Primer Design:
GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):
E. Coli:
http://www.uniprot.org/uniprot/P0AFG3
Wolbachia:
http://www.uniprot.org/uniprot/Q5GSP1